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Impaired bacterial killing in early obstructive jaundice
C E Scott-Conner1, J B Grogan, K S Scher
1Department of Surgery, University of Mississippi School of Medicine, Jackson 39216-4505.
American Journal of Surgery
|September 1, 1993
Summary
Bile duct ligation (BDL) in rats significantly increases susceptibility to Streptococcus pneumoniae infection, leading to higher rates of bacteremia and organ colonization compared to controls. This suggests BDL impairs the host immune response against this common pathogen.
Area of Science:
- Microbiology
- Immunology
- Surgical Research
Background:
- Bile duct ligation (BDL) is a surgical procedure that can lead to complications such as infection.
- Streptococcus pneumoniae is a common bacterial pathogen that can cause severe infections.
Purpose of the Study:
- To investigate the impact of BDL on the host's susceptibility to Streptococcus pneumoniae infection.
- To evaluate the host immune response and bacterial dissemination following BDL and subsequent infection.
Main Methods:
- Sprague-Dawley rats underwent either BDL or sham celiotomy (SC).
- Rats were challenged with intraperitoneal injection of Streptococcus pneumoniae.
- Quantitative bacterial cultures were performed on multiple organs and blood samples 4 hours post-infection.
Main Results:
- 100% of BDL rats exhibited bacteremia, compared to 50% of SC rats (p < 0.05).
- Significantly higher bacterial loads were recovered from the lungs, liver, spleen, and kidneys of BDL rats.
- BDL was associated with impaired bacterial killing and persistent bacteremia.
Conclusions:
- BDL significantly compromises the host's ability to clear Streptococcus pneumoniae infection.
- Impaired bacterial clearance in BDL rats leads to increased dissemination and sustained bacteremia.