Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An evaluation of five commercial immunoassay data analysis software systems

R W Gerlach1, R J White, S N Deming

  • 1Lockheed Environmental Systems & Technologies Co., Las Vegas, Nevada 89119.

Analytical Biochemistry
|July 1, 1993
PubMed
Summary

Commercial immunoassay data analysis software often has deficiencies, especially with poor documentation. Using comparison analyses is crucial to avoid incorrect results and ensure reliable four-parameter calibration model outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Characteristics and Treatment of Patients Diagnosed with Pulmonary Arterial Hypertension: A Real-World Study in the USA, Europe and Japan.

Advances in therapy·2024
Same author

DISCUSSION of the John Mandel paper, The Regression Analysis of Collinear Data.

Journal of research of the National Bureau of Standards (1977)·2021
Same author

Tube flushing and drug administration practice in patients in the community on enteral feeding.

Clinical nutrition ESPEN·2017
Same author

Comparative evaluation of the functional properties of superabsorbent dressings and their effect on exudate management.

Journal of wound care·2016
Same author

Changes in intestinal fluid transport in Atlantic salmon (Salmo salar L) during parr-smolt transformation.

Fish physiology and biochemistry·2013
Same author

Binding and inhibition of protease enzymes, including MMPs, by a superabsorbent dressing in vitro.

Journal of wound care·2013

Area of Science:

  • Biochemistry
  • Medical Technology
  • Data Science

Background:

  • Commercial software is widely used for immunoassay data analysis.
  • Numerous software systems exist, but their reliability and ease of use can vary.
  • Accurate data analysis is critical for reliable immunoassay results.

Purpose of the Study:

  • To evaluate the deficiencies of five commercial software systems for immunoassay data analysis.
  • To assess the usability and accuracy of statistical outputs, particularly with the four-parameter calibration model.
  • To provide recommendations for improving software functionality and data analysis practices.

Main Methods:

  • Five commercial immunoassay data analysis software systems were evaluated.
  • Multiple data sets were analyzed using a four-parameter calibration function in each system.

Related Experiment Videos

  • Results were compared against an independent analysis method to assess accuracy and consistency.

    • Main Results:

    • Significant deficiencies were identified across the evaluated software systems.
    • Poor documentation frequently compromised the utility of statistical outputs.
    • Comparable results were achieved across systems, but multiple analysis attempts were often required.
    • Difficulty in monitoring software options increases the risk of obtaining incorrect results without comparison analyses.

    Conclusions:

    • Commercial immunoassay software requires improvement in functionality and documentation.
    • Users must employ comparison analyses to validate results and mitigate risks of errors.
    • The four-parameter calibration model is a viable option, but its implementation needs careful monitoring within software.