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Activation of mitochondrial DNA replication by He-Ne laser irradiation
R A Vacca1, E Marra, E Quagliariello
1Centro di Studio sui Mitocondrie e Metabolismo Energetico, C.N.R. Bari, Italy.
Abstract:
To gain further insight into the effect of He-Ne laser irradiation on the mitochondrial biosynthetic apparatus, DNA synthesis was measured in mitochondria, mitoplasts and mitochondrial matrix fraction irradiated with low power continuous wave He-Ne laser (energy dose 5 Joules/cm2; laser power 12 mW). As a result of irradiation, the amount of alpha-[32P]dATP incorporated in acid insoluble materials was found to increase. Electrophoretic analysis of in vitro synthesised DNA purified from mitochondria, mitoplasts and matrix fraction shows an increase of 50-60% of DNA synthesis in the irradiated samples. These data show that He-Ne laser stimulates the replication of mitochondrial DNA in both intact organelles and soluble matrix fraction, thus suggesting an interaction of laser light with matrix soluble molecule/s.