Related Experiment Videos
A rapid optimized protocol for downward alkaline Southern blotting of DNA
P A Koetsier1, J Schorr, W Doerfler
1Institute for Genetics, University of Cologne, FRG.
Biotechniques
|August 1, 1993
Abstract:
A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.