Determination of tenoxicam in plasma by high-performance liquid chromatography

A high-performance liquid chromatographic technique with a lower detection limit for measuring tenoxicam plasma levels was standardized. Tenoxicam was extracted with dichloromethane from acidified plasma and the evaporated extracts were analysed on a reversed-phase column using a methanol-phosphate buffer mobile phase and setting ultraviolet detection at 355 nm. A reproducible calibration curve in the 5-2000 ng/ml range was obtained. The mean recovery of extraction was 98.99 +/- 4.3% and the detection limit was 5 ng/ml.