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Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
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[Study of autolytic changes in human myocardial proteins by two-dimensional electrophoresis]

M N Tsvetkova, L I Kovalev, S S Shishkin

    Voprosy Meditsinskoi Khimii
    |July 1, 1993
    PubMed

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    Two-dimensional Gel Electrophoresis01:22

    Two-dimensional Gel Electrophoresis

    Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
    The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
    Biological samples, such as  cells...