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Enzyme rate assay for 5-fluorocytosine
A Shivji1, M Bernstein, M A Noble
1Department of Laboratory Medicine, University Hospital, Vancouver, British Columbia, Canada.
American Journal of Clinical Pathology
|September 1, 1993
Summary
A new enzyme rate assay for 5-fluorocytosine provides rapid results within 10 minutes. This method is also more reliable, unaffected by bilirubin or triglycerides, simplifying clinical laboratory testing.
Area of Science:
- Clinical Chemistry
- Enzyme Kinetics
- Therapeutic Drug Monitoring
Background:
- Traditional enzyme assays for 5-fluorocytosine can be time-consuming.
- Existing methods may be susceptible to interference from sample components like bilirubin and triglycerides.
- Accurate monitoring of 5-fluorocytosine levels is crucial for patient treatment.
Purpose of the Study:
- To develop a faster and more robust enzyme assay for 5-fluorocytosine.
- To reduce interference from common sample analytes.
- To improve the efficiency of 5-fluorocytosine therapeutic drug monitoring.
Main Methods:
- Modified an existing enzyme assay for 5-fluorocytosine from endpoint to rate analysis.
- Evaluated assay performance with varying levels of bilirubin and triglycerides.
- Assessed overall reliability, speed, and cost-effectiveness.
Main Results:
- The modified assay provides results within 10 minutes.
- The rate analysis method significantly reduces interference from bilirubin and triglycerides.
- Eliminates the need for corrections in icteric or lipemic samples.
- Demonstrated consistent reliability and cost-effectiveness.
Conclusions:
- The modified enzyme rate assay is a rapid, reliable, and cost-effective method for 5-fluorocytosine measurement.
- This assay is suitable for routine use in clinical laboratories.
- The reduced susceptibility to interference simplifies sample handling and reporting.