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MCM1 binds to a transcriptional control element in Ty1
1Department of Chemistry, University of North Carolina, Chapel Hill 27599.
Molecular and Cellular Biology
|January 1, 1993
Summary
The MCM1 protein binds to Ty1 elements in yeast, activating gene expression. This binding differs from its role in mating genes, suggesting a distinct function in Ty1-mediated transcription.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Gene Regulation
Background:
- Ty1 transposable elements in Saccharomyces cerevisiae can activate adjacent gene expression.
- Ty1-activated genes share regulatory similarities with yeast mating genes.
Purpose of the Study:
- To investigate the role of the MCM1 protein in Ty1-activated gene expression.
- To determine if MCM1 binding to Ty1 elements is associated with transcriptional activation.
Main Methods:
- Electrophoretic mobility shift assays (EMSA) to detect MCM1 binding to DNA sequences.
- Analysis of gene expression in yeast strains with Ty1 insertions.
- Comparison of MCM1 binding patterns at Ty1 elements versus mating gene promoters.
Main Results:
- The MCM1 protein binds to a transcriptional regulatory sequence within the Ty1 element.
- MCM1 binding to Ty1 correlates with its ability to activate adjacent gene expression.
- Unlike its function at mating gene promoters, MCM1 binds to Ty1 elements independently of coactivators like STE12, alpha 1, or alpha 2.
Conclusions:
- MCM1 plays a significant role in Ty1-activated gene expression in Saccharomyces cerevisiae.
- The mechanism of MCM1 action in Ty1-mediated transcription differs from its role in regulating mating genes, highlighting context-dependent functions.