Aldosterone mediates an increase in [3H]ouabain binding at Na+, K(+)-ATPase sites in the mammalian inner ear

Na+,K(+)-ATPase has been implicated in the maintenance of high [K+], low [Na+] in endolymph of the inner ear, ionic properties considered to support transduction by the receptor cells. In exocrine ion-transporting epithelia, Na+,K(+)-ATPase activity is modulated by aldosterone, a mineralocorticoid hormone. In the present study, the effect of alteration of serum aldosterone levels on Na+,K(+)-ATPase in ion-transporting regions of the mammalian inner ear was investigated. A high Na+/low K+ diet offered ad libitum for 5 days was utilized to significantly decrease serum aldosterone in male Hartley guinea pigs compared to controls. An injection of aldosterone (10 micrograms/100 g b.wt.) 21 h prior to sacrifice resulted in significant elevation of serum aldosterone over that obtained with the high Na+/low K+ diet. Binding of [3H]ouabain, a specific inhibitor of Na+,K(+)-ATPase, was significantly elevated in microdissected lateral wall of the basal turn of the cochlea and in the ampulla of the semicircular canal, for aldosterone-injected vs. vehicle-injected animals. Serum [Na+] and [Cl-] were elevated in animals on the high Na+/low K+ diet and unaltered by administration of exogenous aldosterone. The enhancement of ouabain binding in inner ear tissues observed in aldosterone-injected animals, therefore, did not appear to reflect an alteration of serum electrolytes per se. The results of these experiments are consistent with the hypothesis that aldosterone increases the number of Na+,K(+)-ATPase sites in ion-transporting epithelia of the mammalian cochlea and semicircular canal.