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Polyene antibiotics inhibit superoxide-producing NADPH oxidase in a polymorphonuclear cell-free system
E Capuozzo1, C Crifò, C Salerno
1Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Biochemical Pharmacology
|February 9, 1993
Abstract:
We studied the effect of polyene macrolide antibiotics on the NADPH-dependent superoxide production induced by arachidonic acid in a cell-free system consisting of the membrane and cytosolic fractions obtained from bovine polymorphonuclear leukocytes. Preincubation of the membrane fraction with polyenes before addition of the soluble components of the reaction mixture resulted in a dose-dependent inhibition of superoxide production.