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Complete nucleotide sequence of the cDNA encoding rabbit coagulation factor VII
A B Brothers1, B J Clarke, W P Sheffield
1Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Thrombosis Research
|January 15, 1993
Abstract:
Rabbit coagulation factor VII cDNA has been cloned and sequenced. Two rabbit liver cDNA libraries in lambda gt11 were screened using both random-primed, 32p-labelled, factor VII cDNAs and polymerase chain reaction technology. Three overlapping cDNA clones were isolated comprising a sequence of 1572 base pairs of DNA encoding an open-reading-frame of 443 amino acids. Mature circulating rabbit factor VII is predicted to contain 404 amino acids, two less than its human counterpart and three less than bovine factor VII.