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Related Experiment Videos

Complete nucleotide sequence of the cDNA encoding rabbit coagulation factor VII

A B Brothers1, B J Clarke, W P Sheffield

  • 1Department of Pathology, McMaster University, Hamilton, Ontario, Canada.

Thrombosis Research
|January 15, 1993
PubMed
Summary

Researchers cloned and sequenced rabbit coagulation factor VII cDNA. This study provides the full DNA sequence and amino acid composition of rabbit factor VII, aiding comparative analysis with other species.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Coagulation factor VII (FVII) is a crucial protein in the blood clotting cascade.
  • Understanding FVII structure and function across species aids in comparative hematology and drug development.

Purpose of the Study:

  • To clone and sequence the complementary DNA (cDNA) of rabbit coagulation factor VII.
  • To determine the full genetic sequence and predicted amino acid composition of rabbit FVII.

Main Methods:

  • Screening of two rabbit liver cDNA libraries using lambda gt11.
  • Hybridization with labeled factor VII cDNAs and polymerase chain reaction (PCR) technology.
  • Isolation and sequencing of overlapping cDNA clones.

Main Results:

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  • A 1572 base pair DNA sequence encoding an open-reading-frame of 443 amino acids was identified.
  • Mature circulating rabbit factor VII is predicted to consist of 404 amino acids.
  • Rabbit FVII has a shorter amino acid sequence compared to human and bovine counterparts.

Conclusions:

  • The complete cDNA sequence of rabbit coagulation factor VII has been successfully determined.
  • This provides a foundation for further studies on rabbit FVII structure-function relationships.
  • Comparative analysis with human and bovine FVII can offer insights into evolutionary aspects of coagulation factors.