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Related Experiment Videos

Renal mononuclear cells secrete a factor triggering mesangial cell proliferation

J Weissgarten1, S Berman, Z Averbukh

  • 1Nephrology Division, Assaf Harofeh Medical Center, Zerifin, Israel.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1993
PubMed
Summary

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Following kidney removal surgery, specific immune cells in the remaining kidney (renal mononuclear cells) are activated. These cells secrete growth factors that significantly increase the proliferation of normal mesangial cells.

Area of Science:

  • Nephrology
  • Immunology
  • Cell Biology

Background:

  • Unilateral nephrectomy triggers compensatory renal growth.
  • The role of renal immune cells in compensatory kidney growth is not fully understood.
  • Mesangial cells are crucial for kidney structure and function.

Purpose of the Study:

  • To investigate the role of renal mononuclear cells (RMNC) and peripheral blood mononuclear cells (PBMC) after unilateral nephrectomy.
  • To determine if conditioned media from these cells influence mesangial cell proliferation.
  • To identify potential growth factors involved in kidney regeneration.

Main Methods:

  • Rats underwent unilateral nephrectomy or sham surgery.
  • Renal mononuclear cells (RMNC) and peripheral blood mononuclear cells (PBMC) were isolated and cultured.

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  • Cells were cultured with different sera (control, sham, post-nephrectomy).
  • Conditioned media were used to culture normal mesangial cells.
  • Mesangial cell proliferation was measured.

    • Main Results:

    • Conditioned media from RMNC cultured with post-nephrectomy serum significantly enhanced mesangial cell proliferation.
    • No significant effect on mesangial cell proliferation was observed with other conditioned media (PBMC or control RMNC).
    • This suggests a specific factor secreted by activated RMNC.

    Conclusions:

    • Unilateral nephrectomy activates RMNC in the remaining kidney.
    • Activated RMNC secrete growth factor(s) that stimulate mesangial cell proliferation.
    • These findings highlight a potential mechanism for compensatory renal growth.