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Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method
I Lascu1, K LeBlay, M L Lacombe
1Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
Analytical Biochemistry
|February 15, 1993
Abstract:
A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes.