Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific

R K Dawe1, A R Lachmansingh, M Freeling

  • 1Department of Plant Biology, University of California, Berkeley 94720.

The Plant Cell
|March 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns of polypeptide synthesis in various maize organs under anaerobiosis.

Planta·2013
Same author

Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure.

Plant molecular biology·2013
Same author

An unusually compact retrotransposon in maize.

Plant molecular biology·2013
Same author

Diversity and abundance of the abnormal chromosome 10 meiotic drive complex in Zea mays.

Heredity·2013
Same author

Lax midrib1-O, a systemic, heterochronic mutant of maize.

American journal of botany·2011
Same author

Identification of a maize neocentromere in an oat-maize addition line.

Cytogenetic and genome research·2009
Same journal

The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses.

The Plant cell·2026
Same journal

GIGANTEA shapes diurnal seedling growth by sequestering SMAX1 and SMXL2.

The Plant cell·2026
Same journal

Vascular-specific genome editing enhances low-phosphate tolerance in rice.

The Plant cell·2026
Same journal

The gatekeeper: How PSY1 controls root growth.

The Plant cell·2026
Same journal

OsOSCA2.4 regulates post-Golgi trafficking of storage proteins by modulating Ca2+ homeostasis in rice endosperm.

The Plant cell·2026
Same journal

Age-dependent epigenetic control of flavonoid metabolism underlies chemical defenses in ancient Ginkgo biloba.

The Plant cell·2026
See all related articles

Transposable element excision in maize Alcohol dehydrogenase-1 (Adh1) created new alleles. These altered Adh1 gene expression specifically in pollen, demonstrating plant overexpression mutants.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Gene regulation

Background:

  • The Adh1-Fm335 mutation in maize involves a Dissociation (Ds1) transposable element.
  • Ds1 excision can lead to DNA additions and rearrangements in the Adh1 gene.
  • Understanding gene expression changes after transposable element activity is crucial.

Purpose of the Study:

  • To characterize revertant Adh1 alleles generated by Ds1 excision.
  • To investigate ADH1 activity and gene expression in different maize tissues.
  • To identify mechanisms of pollen-specific gene regulation.

Main Methods:

  • Characterization of 16 revertant Adh1 alleles.
  • Assessing ADH1 activity in scutellum, anaerobic root, and pollen.
  • Quantitative RNase protection assays to measure mRNA levels.

Related Experiment Videos

Main Results:

  • No significant differences in Adh1 expression in sporophytic tissues (scutellum, root).
  • Significant allelic variation in pollen ADH1 expression, including underexpression (48%) and overexpression (163%).
  • Mutant phenotypes correlate with changes in steady-state mRNA levels in pollen.

Conclusions:

  • Demonstration of a plant overexpression mutant.
  • Minor alterations in untranslated leader sequences can cause significant mRNA level increases.
  • Insights into molecular mechanisms of pollen-specific gene expression.