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Ammonia potentiates GABAA response in dissociated rat cortical neurons
K Takahashi1, H Kameda, M Kataoka
1Research Laboratory Roussel Morishita Co., Ltd., Shiga, Japan.
Neuroscience Letters
|March 5, 1993
Abstract:
The ammonium ion (NH4+) evoked no response itself but facilitated the GABA-induced Cl- current (ICl) in dissociated rat cortical neurons. The GABA concentration-response curve shifted parallely to the left without changing the maximum response. Reversal potential of GABA response did not change in the presence of NH4+ at the concentration range between 0.1 and 1 mM. Ro 15-1788, a selective benzodiazepine receptor antagonist, did not affect the facilitatory action of NH4+ on GABAA receptor. The results suggest that NH4+ modifies the affinity of GABAA receptor for GABA, without the involvement of benzodiazepine receptors.