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[ATP formation in a model system with cytochrome c oxidase]
Izvestiia Akademii Nauk. Seriia Biologicheskaia
|March 1, 1993
Summary
Isolated cytochrome c-oxidase can generate ATP, a process linked to oxygen consumption and phosphate changes. This enzyme also exhibits adenylate kinase activity, both influenced by specific inhibitors and activators.
Area of Science:
- Biochemistry
- Enzymology
- Cellular respiration
Context:
- Cytochrome c-oxidase is a key enzyme in the electron transport chain.
- Its role in ATP synthesis has been investigated.
- Understanding its enzymatic activities is crucial for cellular energy metabolism.
Purpose:
- To investigate the ATP-forming ability of isolated cytochrome c-oxidase.
- To characterize the conditions and factors influencing this activity.
- To explore the adenylate kinase activity of cytochrome c-oxidase.
Summary:
- Isolated cytochrome c-oxidase demonstrates ATP synthesis coupled with inorganic phosphate and oxygen uptake.
- This process is stimulated by reduced cytochrome c and inhibited by ammonium sulphate, SF 6847, and oligomycin.
- In the absence of orthophosphate, cytochrome oxidase exhibits adenylate kinase activity, also modulated by reduced cytochrome c and inhibitors.
Impact:
- Reveals a novel ATP-generating capability of cytochrome c-oxidase.
- Provides insights into the complex enzymatic functions of cytochrome c-oxidase beyond electron transport.
- Contributes to a deeper understanding of cellular energy production mechanisms.