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Related Experiment Videos

ATP depletion causes a reversible decrease in Na+ pump density in cultured ventricular myocytes

H Ikenouchi1, L Zhao, M McMillan

  • 1Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132.

The American Journal of Physiology
|April 1, 1993
PubMed
Summary
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Prolonged ATP depletion impairs cardiac cell function by reducing sodium pump density. Fetal calf serum (FCS) aids recovery, restoring potassium homeostasis and cell contractility.

Area of Science:

  • Cardiovascular Physiology
  • Cellular Metabolism
  • Ion Homeostasis

Background:

  • Prolonged but sublethal ATP depletion can disrupt cellular function.
  • Understanding the factors contributing to impaired potassium (K+) homeostasis is crucial for cardiac health.

Purpose of the Study:

  • To investigate the effects of metabolic inhibition on chick ventricular myocytes.
  • To identify factors influencing the recovery of K+ homeostasis and cellular function after ATP depletion.

Main Methods:

  • Cultured chick ventricular myocytes were subjected to metabolic inhibition using 2-deoxy-D-glucose and NaCN.
  • Recovery was assessed in media with or without fetal calf serum (FCS).
  • Measurements included spontaneous contractions, K+ content and uptake, membrane potential, and Na+ pump density.

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Main Results:

  • Metabolic inhibition acutely decreased Na+ pump site density.
  • Recovery in serum-free medium resulted in persistently depressed K+ uptake, K+ content, membrane depolarization, reduced cell number, and failed contractions.
  • Recovery in FCS-containing medium restored Na+ pump density, K+ uptake, K+ content, cell number, and spontaneous contractions.

Conclusions:

  • Sublethal ATP depletion severely impairs cardiac myocyte function and K+ homeostasis.
  • Fetal calf serum is essential for the recovery of Na+ pump density, K+ homeostasis, and cellular function in myocytes recovering from metabolic stress.