Pituitary adenylate cyclase activating polypeptide receptors are present on small cell lung cancer cells

¹Department of Biochemistry and Molecular Biology, George Washington University School of Medicine and Health Sciences, Washington, DC 20037.

|March 1, 1993

Abstract:

The effect of pituitary adenylate cyclase activating polypeptide (PACAP) on small cell lung cancer (SCLC) cells was investigated. In Fura 2-AM loaded NCI-N417 cells, PACAP-27 or PACAP-38 elevated cytosolic calcium in a dose-dependent manner. The cytosolic calcium was elevated from 133 to 185 nM with PACAP-27 (100 nM). Because PACAP-27 strongly elevated cytosolic calcium after the addition of 1 mM EGTA, PACAP released calcium from intracellular pools using NCI-N417 cells; similar results were obtained for SCLC cell line NCI-H345. Pituitary adenylate cyclase activating polypeptide-27 inhibited 125I-VIP and 125I-PACAP binding to NCI-N417 cells with high affinity (IC50 = 30 and 5 nM), whereas VIP inhibited 125I-VIP and 125I-PACAP-27 binding with IC50 values of 5 and 200 nM. Both 100 nM VIP and PACAP-27 elevated cAMP levels in NCI-H345 cells. In contrast, 1 microM VIP had no effect on cytosolic calcium, whereas 100 nM PACAP-27 caused a strong calcium response. Both 100 nM VIP and 10 nM PACAP-27 significantly stimulated the clonal growth of NCI-H345 cells. These data suggest that biologically active VIP and PACAP receptors are present on SCLC cells.

Related Experiment Videos

Pituitary adenylate cyclase activating polypeptide receptors are present on small cell lung cancer cells

Related Concept Videos

Assessment of agreement and interchangeability between the TEG5000 and TEG6S thromboelastography haemostasis analysers: a prospective validation study.

The effect of a perioperative ketamine infusion on the incidence of chronic postsurgical pain-a pilot study.

Successful treatment of severe thrombocytopenia with romiplostim in a pregnant patient with systemic lupus erythematosus.

Modified Hodge test: A simple and effective test for detection of carbapenemase production.

Isolation, identification and biological activity of gastrin-releasing peptide 1-46 (oGRP 1-46), the primary GRP gene-derived peptide product of the pregnant ovine endometrium.

Autoradiographic localization of neuromedin B binding sites in rat brain.

Galanin signaling in stress-induced brain-gut axis dysregulation: Receptor-specific mechanisms and context-dependent pharmacology.

KR-36996, an orally active urotensin II receptor antagonist, attenuates hepatic inflammation and fibrosis in methionine- and choline-deficient diet-induced fibrosing steatohepatitis.

Phoenixin-14 attenuates pancreatic injury by modulating inflammation, oxidative stress, and apoptosis in L-arginine-induced acute pancreatitis.

Endogenous opiates and behavior: 2025.

Sulfakinin neuropeptides and their receptors in insects: Molecular signalling, physiological diversity, and pest management implications.

Altered gastrointestinal function and neurotensin signaling in experimental type 2 diabetes.

Individualized breast cancer survival prediction in clinical practice: a SEER-derived interactive web tool integrating molecular and anatomical factors.

A Greek Real-World Experience With the Implementation of the EndoPredict Gene Signature in a Cohort of Pre-menopausal and Post-menopausal Patients With ER+/HER2- Breast Cancer.

A Myeloid-Specific Endogenous TRP1-CAR Knock-in Mouse Model for Melanoma-Targeted Immunotherapy.

Clinicopathological Features and Statistical Associations in Squamous Cell Carcinoma of the Lip.

Sex-Specific Predictive Value of Obesity-Related Indicators for Diabetic Kidney Disease in Type 2 Diabetes Mellitus.

Prognostic Value of FST and Relevance to Immune Infiltration in Head and Neck Squamous Cell Carcinoma.

Related Experiment Videos

Pituitary adenylate cyclase activating polypeptide receptors are present on small cell lung cancer cells

Abstract:

Related Concept Videos

Related Articles