Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein-radical enzymes

J Z Pedersen1, A Finazzi-Agrò

  • 1Department of Biology, Tor Vergata University of Rome, Italy.

FEBS Letters
|June 28, 1993
PubMed
Summary
This summary is machine-generated.

Protein-radical enzymes utilize intrinsic amino acid radicals as cofactors. These radicals act as one-electron gates, managing electron transfer in biological processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carmine Melino and his love for the history of hygiene.

Annali di igiene : medicina preventiva e di comunita·2017
Same author

Erythrocyte glutathione transferase: a general probe for chemical contaminations in mammals.

Cell death discovery·2016
Same author

Metabolic effects of TiO2 nanoparticles, a common component of sunscreens and cosmetics, on human keratinocytes.

Cell death & disease·2013
Same author

Electron spin resonance characterization of the radicals produced by enzymatic or chemical cleavage of vicine.

Biochemistry·2009
Same author

Evidence for the intracellular accumulation of anandamide in adiposomes.

Cellular and molecular life sciences : CMLS·2008
Same author

Biochemical changes in endocannabinoid system are expressed in platelets of female but not male migraineurs.

Cephalalgia : an international journal of headache·2006
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Area of Science:

  • Biochemistry
  • Enzymology
  • Molecular Biology

Background:

  • Protein-radical enzymes are a class of enzymes that employ a radical cofactor.
  • This radical is derived from an intrinsic amino acid residue within the enzyme's active site.

Purpose of the Study:

  • To elucidate the mechanistic role of radical cofactors in protein-radical enzymes.
  • To categorize the types of amino acid residues involved in radical formation.

Main Methods:

  • Review of existing literature on protein-radical enzymes.
  • Analysis of amino acid residues known to form radicals in various enzyme families.

Main Results:

  • Identified tyrosine, modified tyrosine, tryptophan, modified tryptophan, and glycine as amino acids forming radical cofactors.

Related Experiment Videos

  • Examples include ribonucleotide reductase, photosystem II, prostaglandin H synthase, amine oxidase, galactose oxidase, cytochrome c peroxidase, methylamine dehydrogenase, and pyruvate formate lyase.
  • The primary mechanistic role identified is that of a one-electron gate.

    • Conclusions:

    • Protein-radical enzymes utilize a diverse range of amino acid residues to generate radical cofactors.
    • These radical cofactors function as crucial one-electron gates, facilitating controlled electron transfer in enzymatic reactions.