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Updated: Aug 6, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
A flower senescence-related mRNA from carnation encodes a novel protein related to enzymes involved in phosphonate
H Wang1, A S Brandt, W R Woodson
1Department of Horticulture, Purdue University, West Lafayette, IN 47907-1165.
Abstract:
We have isolated a cDNA clone (pSR132) representing a mRNA which accumulates in senescing carnation flower petals in response to ethylene. In vitro translation of RNA selected by hybridization with pSR132 indicated the mRNA encoded a polypeptide of approximately 36 kDa. This was confirmed by DNA sequence analysis, which predicted a peptide composed of 318 amino acids with a calculated molecular weight of 34.1 kDa. Comparison of the predicted peptide sequence of pSR132 with other proteins compiled in the NBRF data base revealed significant homology with carboxyphosphonoenolpyruvate mutase and phosphoenolpyruvate mutase from Streptomyces hygroscopicus and Tetrahymena pyriformis, respectively. These enzymes are involved in the formation of C-P bonds in the biosynthesis of phosphonates. C-P bonds are found in a wide range of organisms, but their presence or formation in higher plants has not been investigated.
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