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Characterization of in vivo phosphorylation of activin type II receptor
H Ichijo1, H Yamashita, P ten Dijke
1Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Biochemical and Biophysical Research Communications
|August 16, 1993
Abstract:
An antiserum was generated against a C-terminal peptide of the mouse activin type II receptor (ActR-II), which specifically immunoprecipitates ActR-II protein transiently expressed in COS-1 cells. Autophosphorylation activity of the ActR-II was examined in [32P]orthophosphate labeled COS-1 cells. The immunoprecipitated ActR-II protein was found to be phosphorylated on serine residues in the absence of ligand stimulation, and the phosphorylation was slightly increased after stimulation with activin A.