Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear phosphoinositidase C during growth factor stimulation

L Cocco1, A M Martelli, S Capitani

  • 1Institute of Human Anatomy, University of Bologna, Italy.

Advances in Enzyme Regulation
|January 1, 1993
PubMed
Summary

Phosphoinositidase C beta is found in the nucleus of Swiss 3T3 cells and activated by Insulin-like Growth Factor I, indicating a distinct nuclear signaling system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diet Modulation Restores Autophagic Flux in Damaged Skeletal Muscle Cells.

The journal of nutrition, health & aging·2019
Same author

INPP4B overexpression and c-KIT downregulation in human achalasia.

Neurogastroenterology and motility·2018
Same author

Therapeutic targeting of CK2 in acute and chronic leukemias.

Leukemia·2017
Same author

Therapeutic potential of targeting sphingosine kinases and sphingosine 1-phosphate in hematological malignancies.

Leukemia·2016
Same author

Feedbacks and adaptive capabilities of the PI3K/Akt/mTOR axis in acute myeloid leukemia revealed by pathway selective inhibition and phosphoproteome analysis.

Leukemia·2014
Same author

Activity of the pan-class I phosphoinositide 3-kinase inhibitor NVP-BKM120 in T-cell acute lymphoblastic leukemia.

Leukemia·2013

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Phosphoinositidase C (PLC) enzymes play crucial roles in cellular signaling.
  • Previous research identified PLC gamma in the cytoplasm, but nuclear localization and function were less understood.

Purpose of the Study:

  • To investigate the presence and function of phosphoinositidase C beta (PLCβ) in the nucleus of Swiss 3T3 cells.
  • To determine if nuclear PLCβ is activated by growth factors and contributes to nuclear signaling.

Main Methods:

  • Subcellular fractionation to isolate nuclear and cytoplasmic components.
  • Western blotting to detect specific PLC isoforms.
  • Stimulation of Swiss 3T3 fibroblasts with Insulin-like Growth Factor I (IGF-I).

Related Experiment Videos

Main Results:

  • Phosphoinositidase C beta (PLCβ) is specifically localized to the nucleus in Swiss 3T3 cells.
  • The gamma isoform (PLCγ) is confined to the cytoplasm.
  • IGF-I treatment induces rapid, transient activation of nuclear PLCβ, while cytoplasmic PLC remains unaffected.
  • This activation correlates with a decrease in nuclear polyphosphoinositide lipids.

Conclusions:

  • A distinct nuclear polyphosphoinositide signaling system exists, separate from the plasma membrane system.
  • This nuclear system involves specific lipid kinases and phosphoinositidase C beta (PLCβ).
  • Nuclear PLCβ activation by IGF-I is a key event in nuclear lipid signaling.