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Mucin-producing pancreatic tumors: a study of nuclear DNA content by flow cytometry
Y Murakami1, T Yokoyama, T Kodama
1First Department of Surgery, Hiroshima University School of Medicine, Japan.
Abstract:
Nuclear DNA content in eight surgically resected mucin-producing pancreatic tumors (MPPT) consisting of two mucinous intraductal adenocarcinomas (MIDAC), two mucinous intraductal adenomas (MIDA), one mucinous cyst-adenocarcinoma (MCAC), and three mucinous cystadenomas (MCA) were measured by flow cytometry using paraffin-embedded tissue samples. The technique of Shutte was used for the preparation of paraffin-embedded tissue into single dissociated nuclei, while the method of Vindelov was used for staining the isolated nuclei with propidium iodine. Clinicopathologically, the four patients with MIDAC or MIDA were all male and had cystic lesions with a dilated pancreatic duct at the head of the pancreas, while the four patients with MCAC or MCA were all females and had cystic tumors at either the body or tail of the pancreas. All eight patients with MPPT had no metastasis to the regional lymph nodes and were all still alive without recurrence. In an analysis of nuclear DNA content, seven of eight patients had DNA diploid tumors while one patient with a MIDAC perforating the duodenum and choledochus had a DNA aneuploid tumor. Thus, these findings suggest that DNA diploid patterns in MPPT might be associated with a favorable prognosis in MPPT although some patients whose MPPT invaded the surrounding organs might have DNA aneuploid tumors.
Insights
Nuclear DNA content analysis in mucin-producing pancreatic tumors (MPPT) revealed that most tumors were DNA diploid, suggesting a favorable prognosis. A DNA aneuploid tumor was observed in one advanced case.
Area of Science:
- Oncology
- Pathology
- Genetics
Background:
- Mucin-producing pancreatic tumors (MPPT) encompass a spectrum of neoplastic lesions.
- Accurate prognostic markers for MPPT are crucial for clinical management.
Purpose of the Study:
- To investigate the correlation between nuclear DNA content and clinicopathological features in MPPT.
- To assess the prognostic value of DNA ploidy in MPPT.
Main Methods:
- Flow cytometry was employed to measure nuclear DNA content in paraffin-embedded MPPT samples.
- Tissue preparation involved single nucleus dissociation (Shutte technique) and propidium iodide staining (Vindelov method).
Main Results:
- Seven of eight MPPT cases exhibited DNA diploid tumors.
- One mucinous intraductal adenocarcinoma (MIDAC) with duodenal and choledochal perforation showed DNA aneuploidy.
- Clinicopathological differences were noted between tumor subtypes and locations, with gender and pancreatic region variations observed.
Conclusions:
- DNA diploidy in MPPT appears associated with a favorable prognosis.
- DNA aneuploidy may occur in MPPT with extensive local invasion.