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Related Experiment Videos

Humanized antibodies

G Winter1, W J Harris

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Immunology Today
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

Hybridoma technology produced rodent antibodies with limited use. Protein engineering now creates humanized antibodies, improving treatments for diseases like cancer and autoimmune disorders.

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Area of Science:

  • Biotechnology
  • Immunology
  • Protein Engineering

Background:

  • Hybridoma technology allowed rodent monoclonal antibody production against human targets.
  • Rodent antibodies had limited clinical application due to issues like immunogenicity.
  • Advancements in protein engineering are crucial for therapeutic antibody development.

Purpose of the Study:

  • To review the evolution of antibody technology from hybridomas to humanized antibodies.
  • To highlight the clinical advantages of humanized antibodies in various diseases.
  • To underscore the impact of protein engineering on antibody-based therapeutics.

Main Methods:

  • Review of hybridoma technology for monoclonal antibody generation.
  • Analysis of protein engineering techniques for antibody humanization.

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  • Examination of clinical data for humanized antibody efficacy and safety.

    • Main Results:

    • Humanized antibodies demonstrate improved pharmacokinetic profiles compared to rodent antibodies.
    • Reduced immunogenicity of humanized antibodies enhances patient tolerance and treatment effectiveness.
    • Successful clinical applications of humanized antibodies in treating infectious diseases, autoimmune disorders, and cancers.

    Conclusions:

    • Protein engineering has overcome limitations of rodent antibodies, enabling effective humanized antibody therapeutics.
    • Humanized antibodies represent a significant advancement in treating a range of serious diseases.
    • The clinical utility of antibodies has been substantially enhanced through humanization strategies.