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Phenotypic and phylogenetic evidence for a close relationship between Lactococcus garvieae and Enterococcus
A Doménech1, J Prieta, J F Fernández-Garayzábal
1Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Microbiologia (Madrid, Spain)
|April 1, 1993
Summary
Enterococcus seriolicida should be reclassified as Lactococcus based on genetic and biochemical analyses. Both Lactococcus garvieae and this reclassified species exhibit beta-haemolytic activity, challenging prior research.
Area of Science:
- Microbiology
- Fish Pathology
- Bacterial Taxonomy
Background:
- Lactococcus garvieae is a significant pathogen in rainbow trout aquaculture.
- Enterococcus seriolicida has been previously identified as a fish pathogen.
- Previous classifications and characteristics of these bacteria require further investigation.
Purpose of the Study:
- To perform detailed comparative analyses of L. garvieae and E. seriolicida ATCC 49156.
- To clarify the taxonomic position of E. seriolicida ATCC 49156.
- To investigate the haemolytic properties of both bacterial species.
Main Methods:
- Cultural, biochemical, and protein profiling of bacterial strains.
- 16S rRNA gene sequence analysis for phylogenetic identification.
- Assessment of beta-haemolytic activity.
Main Results:
- 16S rRNA sequence analyses confirmed that E. seriolicida ATCC 49156 belongs to the genus Lactococcus.
- Both L. garvieae and the reclassified E. seriolicida demonstrated beta-haemolytic activity.
- Findings contradict previous reports regarding the haemolytic nature of these bacteria.
Conclusions:
- Enterococcus seriolicida ATCC 49156 should be taxonomically reclassified as a species within the Lactococcus genus.
- The beta-haemolytic characteristic is common to both L. garvieae and the reclassified E. seriolicida.
- This study refines the understanding of Lactococcus species relevant to fish health.