Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

D-amino acid levels in human physiological fluids

D W Armstrong1, M Gasper, S H Lee

  • 1Department of Chemistry, University of Missouri-Rolla 65401.

Chirality
|January 1, 1993
PubMed
Summary

Free D-amino acids are present in all human physiological fluids, with levels varying significantly. Urine typically shows the highest concentrations, while amniotic fluid and cerebrospinal fluid (CSF) have the lowest.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of D-amino acid levels in human urine and in commercial L-amino acid samples.

Amino acids·2013
Same author

Factors controlling the level and determination of D-amino acids in the urine and plasma of laboratory rodents.

Amino acids·2013
Same author

Prevention of retinal light damage by zinc oxide combined with rosemary extract.

Molecular vision·2013
Same author

N-acetylcysteine amide, a thiol antioxidant, prevents bleomycin-induced toxicity in human alveolar basal epithelial cells (A549).

Free radical research·2013
Same author

N-acetylcysteine amide protects against methamphetamine-induced tissue damage in CD-1 mice.

Human & experimental toxicology·2012
Same author

Mechanism of signal suppression by anionic surfactants in capillary electrophoresis-electrospray ionization mass spectrometry.

Analytical chemistry·2011

Area of Science:

  • Biochemistry
  • Physiology
  • Analytical Chemistry

Background:

  • D-amino acids, enantiomers of common L-amino acids, are increasingly recognized for their biological roles.
  • Their presence and levels in human physiological fluids are not fully characterized.
  • Understanding D-amino acid distribution is crucial for metabolic and physiological studies.

Purpose of the Study:

  • To investigate the presence and quantify the levels of free D-amino acids in various human physiological fluids.
  • To compare the distribution patterns of D-amino acids across different body compartments.
  • To identify any specific D-amino acids with unique excretion profiles.

Main Methods:

  • Analysis of plasma, urine, cerebrospinal fluid (CSF), and amniotic fluid.

Related Experiment Videos

  • Utilized quantitative methods to determine the concentrations of free D-amino acids.
  • Compared D-amino acid levels relative to their corresponding L-amino acid enantiomers.

    • Main Results:

    • D-amino acids were detected in all tested physiological fluids.
    • Urine exhibited the highest D-amino acid levels, ranging from tenth to low percent of L-amino acids.
    • Amniotic fluid and CSF showed the lowest D-amino acid concentrations (<1% of L-amino acids).
    • D-pipecolic acid was uniquely excreted primarily as the D-enantiomer (>90%), with lower levels in plasma.
    • Observed trends in human fluids showed analogies to recent rodent studies.

    Conclusions:

    • Human physiological fluids contain measurable levels of free D-amino acids.
    • Significant variations in D-amino acid concentrations exist across different fluids, with urine as a major site of excretion.
    • D-pipecolic acid displays a distinct enantiomeric excretion pattern.
    • Findings suggest conserved physiological mechanisms for D-amino acid handling across species.