Effect of alginate and calcium on secretion of IL-1 by immobilized Swiss peritoneal macrophages

M A Tahri Jouti1, H Ramdi, M Lièvremont

  • 1Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée (L.I.R.B.A.) E.N.S. de Cachan, France.

Murine peritoneal macrophages were immobilized in calcium alginate gel macrobeads, using 1.5% Na-alginate and 50 mM CaCl2. Secretion of IL-1 by immobilized macrophages increased during time and reached 10 fold than IL-1 quantities secreted by adherent macrophages. Calcium and alginate individually enhance production of IL-1 by macrophages and act in synergy when macrophages are immobilized in calcium alginate matrix.

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