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In vitro fertilization and embryonic development of oocytes fertilized by sperm treated with 2-deoxyadenosine
D A Imoedemhe1, A B Sigue, E L Pacpaco
1Human Reproductive Biology Unit, Soliman Fakeeh Hospital, Jeddah, Saudi Arabia.
Objective:
To test the effect in an assisted pregnancy program of an agent known to enhance sperm motility and oocyte penetration ability.
Design:
Prospective with internal control.
Setting:
Hospital clinic.
Patients And Interventions:
Forty-two oocytes obtained from women undergoing IVF were inseminated with their husband's sperm washed, incubated, and capacitated in Earle's medium supplemented with 1 mM, 2.5 mM, or 5 mM 2-deoxyadenosine (2-DXA). The outcome of insemination was compared with those of 234 control oocytes from the same women that were inseminated with husband's sperm washed in 2-DXA-free medium. 15, 14, and 13 oocytes were inseminated with sperm washed in 1 mM, 2.5 mM, and 5 mM 2-DXA, respectively.
Results:
Fertilization rates in the respective groups were 93%, 85.7%, and 85.5%. These were higher than the 70.1% fertilization rate in the control group, but only statistically higher (P < .002) in the 1 mM 2-DXA group. Embryonic development in all three 2-DXA groups was comparable to the controls.
Conclusion:
It is suggested that the value of sperm motility enhancing agents requires further evaluation in assisted pregnancy programs.