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Updated: Aug 15, 2026

Immunostaining to Visualize Murine Enteric Nervous System Development
Published on: April 29, 2015
A novel cholinergic-specific antigen (Chol-2) in mammalian brain
E A Derrington1, S Kelić, V P Whittaker
1Arbeitsgruppe Neurochemie, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
Abstract:
Three new antisera have been raised in sheep against cholinergic electromotor presynaptic plasma membranes prepared from the electric organs of the electric ray, Torpedo marmorata. They all recognized one or more cholinergic-specific antigens in the mammalian nervous system by the following criteria: they sensitized the cholinergic subpopulation of rat-brain synaptosomes--and only this subpopulation--to lysis by the complement system and, in an immunocytochemical study, selectively stained choline acetyltransferase-positive cholinergic neurons in the rat spinal cord. However, two of the three antisera failed to recognize Chol-1 alpha and -beta, two closely related minor gangliosides already identified as the cholinergic-specific antigens recognized by previous anti-Torpedo presynaptic plasma membrane antisera or indeed any other ganglioside and the third recognized only Chol-1 alpha. A further investigation of the antigen(s) recognized by the most antigenic of the new antisera indicated that it is proteinaceous in nature, but has epitopes in common with electric organ gangliosides.
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