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Related Experiment Videos

Basic fibroblast growth factor in rat salivary glands

O Amano1, Y Yoshitake, K Nishikawa

  • 1Department of Anatomy, School of Medicine, Kanazawa University, Ishikawa, Japan.

Cell and Tissue Research
|September 1, 1993
PubMed
Summary

Basic fibroblast growth factor (bFGF) stimulates endothelial cell growth and is found in rat salivary gland ducts and specific cells. Its localization differs from other growth factors within these glands.

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Area of Science:

  • Cell Biology
  • Histology
  • Endocrinology

Background:

  • Basic fibroblast growth factor (bFGF) is a potent mitogen involved in cell growth and tissue repair.
  • Salivary glands play crucial roles in oral health and have complex ductal systems.
  • Understanding growth factor localization in salivary glands can reveal insights into gland function and regeneration.

Purpose of the Study:

  • To investigate the presence and cellular distribution of bFGF in rat salivary glands.
  • To assess the biological activity of salivary gland-derived bFGF on endothelial cells.
  • To compare the subcellular localization of bFGF with other growth factors in salivary gland ducts.

Main Methods:

  • Monoclonal antibody production for specific bFGF detection.

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  • Radioimmunoassay (RIA) for quantifying bFGF concentration.
  • Immunocytochemistry and electron microscopy for cellular and subcellular localization.

    • Main Results:

    • Rat salivary gland extracts exhibited significant endothelial cell growth-stimulating activity, neutralized by anti-bFGF antibody.
    • bFGF concentration in submandibular/sublingual glands was comparable to brain levels.
    • Immunoreactivity for bFGF was primarily in epithelial cells of striated and excretory ducts across parotid, sublingual, and submandibular glands.
    • Intense bFGF staining was noted in the granular convoluted tubule's agranular pillar cells of the submandibular gland.
    • Electron microscopy showed diffuse cytoplasmic and nuclear bFGF distribution, excluding secretory granules and organelles.

    Conclusions:

    • bFGF is present and biologically active in rat salivary glands.
    • bFGF exhibits a distinct cellular and subcellular localization pattern within the salivary gland duct system.
    • This unique localization suggests specialized roles for bFGF in salivary gland physiology, separate from other growth factors.