Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression during imaginal disc regeneration detected using enhancer-sensitive P-elements

W J Brook1, L M Ostafichuk, J Piorecky

  • 1Department of Genetics, University of Alberta, Edmonton, Canada.

Development (Cambridge, England)
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Determined Motivation for Alcohol Use and Drinking Frequency, Intensity, and Consequences.

Substance use & misuse·2024
Same author

Tobacco and cannabis use as moderators of the association between physical activity and alcohol use across the adult lifespan in the United States: NHANES, 2005-2016.

Preventive medicine·2021
Same author

Pattern formation in a ts-cell-lethal mutant ofDrosophila: The range of phenotypes induced by larval heat treatments.

Wilhelm Roux's archives of developmental biology·2017
Same author

Clinical Applications of Cine Balanced Steady-State Free Precession MRI for the Evaluation of the Subarachnoid Spaces.

Clinical neuroradiology·2015
Same author

The role of circulating 25 hydroxyvitamin D in asthma: a systematic review.

Allergy·2015
Same author

The impact of anti-inflammatory cytokines on the pancreatic β-cell.

Islets·2014
Same journal

Dissecting planar and vertical organiser signals in early chick neural development.

Development (Cambridge, England)·2026
Same journal

Real-time transcriptomic profiling of hPSC-derived cartilage during development identifies a key role for the extracellular matrix in homeostasis and protection.

Development (Cambridge, England)·2026
Same journal

In preprints - housekeeping the housekeeping genes.

Development (Cambridge, England)·2026
Same journal

In preprints - light, cluster, friction: a cell dance on the gastrulation stage.

Development (Cambridge, England)·2026
Same journal

PBX-dependent and -independent Hox programs establish and maintain motor neuron terminal identity.

Development (Cambridge, England)·2026
Same journal

NUDT21 regulates 3'UTR dynamics in epididymal principal cells to preserve sperm integrity.

Development (Cambridge, England)·2026
See all related articles

Researchers identified genes involved in Drosophila imaginal disc regeneration by screening for altered lac-z expression. This revealed a significant transcriptional response during early regeneration, with some genes potentially specific to this process.

Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Genetics

Background:

  • Drosophila imaginal discs can regenerate missing tissue when cultured in vivo.
  • Regeneration involves cell proliferation forming a blastema, with pattern specification likely mediated by cell interactions.

Purpose of the Study:

  • To identify genes regulating imaginal disc regeneration.
  • To understand the transcriptional events during early regeneration stages.

Main Methods:

  • Screened over 800 enhancer-sensitive P-element insertions for altered lac-z expression in regenerating discs.
  • Used a temperature-sensitive cell-lethal mutation to induce cell death and trigger regeneration.
  • Performed secondary screens to refine gene candidates based on temporal and spatial lac-z expression patterns.

Related Experiment Videos

Main Results:

  • Identified 23 lines with lac-z expression correlated with regeneration blastema formation.
  • Observed a substantial transcriptional response during early imaginal disc regeneration.
  • Found gene expression patterns including regeneration-specific genes, developmentally recruited genes, and genes at compartment boundaries (e.g., decapentaplegic, crumbs).

Conclusions:

  • A significant number of genes are transcriptionally regulated during Drosophila imaginal disc regeneration.
  • The study provides insights into genes potentially exclusive to regeneration and those recruited from earlier developmental programs.
  • Expression patterns suggest cell communication plays a role in regeneration blastema formation.