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Chimeric analysis of T (Brachyury) gene function

V Wilson1, P Rashbass, R S Beddington

  • 1Centre for Genome Research, University of Edinburgh, UK.

Development (Cambridge, England)
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

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Investigating the T (Brachyury) gene function in mouse embryos revealed that T/T cells accumulate in caudal regions, leading to severe tail abnormalities or developmental arrest. This suggests T gene

Area of Science:

  • Developmental Biology
  • Genetics
  • Embryology

Background:

  • The T (Brachyury) gene is crucial for embryonic development.
  • Previous studies indicated T gene involvement in early embryonic stages.
  • Understanding T gene function is key to deciphering developmental processes.

Purpose of the Study:

  • To investigate the function of the T (Brachyury) gene during mid-gestation embryonic development.
  • To analyze the role of T gene in cell migration and tissue formation using chimeric analysis.

Main Methods:

  • Chimeric analysis of mouse embryos (9.5-11.5 days post coitum).
  • Introduction of T gene homozygous/heterozygous embryonic stem (ES) cells into wild-type host embryos.
  • Morphological scoring and assessment of cell colonization in resulting chimeras.

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Main Results:

  • T/T cells showed a significant bias towards caudal regions (tail and allantois).
  • Presence of T/T cells in the allantois disrupted placental connection, causing developmental arrest.
  • T/T cells predominantly in the tail led to severe tail abnormalities (foreshortening, branching, cavities).

Conclusions:

  • The T (Brachyury) gene plays a critical role in the proper deployment of cells emerging from the tailbud.
  • Altered migration or adhesion properties of T/T cells likely cause caudal accumulation.
  • T gene function is essential for normal tail development and placental formation.