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Different target-site specificities of the hairpin ribozyme in cis and trans cleavages
K Fujitani1, N Sasaki-Tozawa, Y Kikuchi
1Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
FEBS Letters
|September 27, 1993
Abstract:
The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.