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Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome
K Tabata1, T Kosuge, T Nakahara
1Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
FEBS Letters
|September 27, 1993
Abstract:
The physical map of the chromosome of Thermus thermophilus HB27 was constructed using three restriction enzymes; EcoRI, SspI and MunI by applying pulsed-field gel electrophoresis techniques. Although the genome size of 1.82 Mb was almost the same as that (1.74 Mb) reported for T. thermophilus HB8 [Borges, K.M., and P.L. Bergquist. (1993) J. Bacteriol. 175, 103-110], the MunI cleavage maps were different. A 240 kb plasmid was detected in HB27, and its physical map was also constructed. In addition, several genes were located on the chromosomal physical map.