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Modulation of the ATP induced [Ca2+]c increase in AS-30D hepatoma cells
M E Torres-Márquez1, S Mejia, R Moreno-Sánchez
1Departamento de Bioquímica, Instituto Nacional de Cardiología, México, D.F.
The International Journal of Biochemistry
|August 1, 1993
Abstract:
1. The regulation of the increase in the cytosolic calcium concentration ([Ca2+]c) induced by extracellular ATP in AS-30D hepatoma cells was studied. 2. Homologous desensitization involving the refilling of intracellular calcium pools and the participation of protein kinase C was found. 3. Isoproterenol, forskolin and dibutyryl-cyclic AMP also induced an increase in [Ca2+]c. 4. Interestingly, synergism was found for isoproterenol or forskolin and ATP. 5. The results suggest that there are two pathways for mobilizing [Ca2+]c in AS-30D hepatoma cells; one is activated by ATP receptors and the other by cyclic AMP.