MchAI and MchAII, two class-II restriction endonucleases from Mycobacterium chelonei
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Updated: Nov 7, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
Abstract:
We have purified and characterized two class-II restriction endonucleases from the saprophyte Mycobacterium chelonei AMB 82. MchAI was an isoschizomer of NotI recognizing and cleaving at 5'-GC/GGCCGC. MchAI did not require Triton X-100 for activity and was fully active at concentrations over 10-200 mM KCl and MgCl2. MchAII was an isoschizomer of HaeIII recognizing and cleaving at 5'-GG/CC. MchAII was active in 10 mM Tris.HCl pH 8.0 containing MgCl2 at 10-200 mM, was strongly inhibited by NaCl at concentrations higher than 10 mM and was inactive at KCl concentrations lower than 250 mM.
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