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Particulate nitrocellulose as a solid phase for protein immobilization in immuno-affinity chromatography
P Hammerl1, A Hartl, J Thalhamer
1University of Salzburg, Department of Biochemistry, Austria.
Journal of Immunological Methods
|September 27, 1993
Summary
Nitrocellulose paper powder (NCP) offers a cost-effective matrix for protein immobilization in immunoaffinity chromatography. This method effectively purifies antibodies and removes unwanted proteins, presenting a viable alternative to existing chromatographic media.
Area of Science:
- Biochemistry
- Chromatography
- Materials Science
Background:
- Protein immobilization is crucial for various biochemical applications, including affinity chromatography.
- Traditional matrices can be expensive or complex to use.
Purpose of the Study:
- To evaluate nitrocellulose paper powder (NCP) as a novel matrix for protein immobilization.
- To assess NCP's utility in immunoaffinity chromatography for antibody purification.
Main Methods:
- Nitrocellulose paper was processed into a powder (NCP) and fractionated by particle size.
- Protein binding capacity and dynamics were studied using bovine serum albumin (BSA).
- NCP was applied in batch and column chromatography for antibody purification and removal.
Main Results:
- NCP demonstrated favorable flow properties comparable to Sephadex G-50.
- Effective protein binding and purification of antibodies were achieved using NCP.
- Batch and column chromatography applications showed successful antibody purification and removal.
Conclusions:
- Nitrocellulose paper powder (NCP) is a cost-effective and versatile matrix for protein immobilization.
- NCP chromatography presents a practical alternative for immunoaffinity purification and protein removal applications.