Theoretical examination of the mechanism of aldose-ketose isomerization

Two mechanisms for an aldose-ketose isomerization have been examined using high level ab initio and semiempirical molecular orbital methods. The proton transfer pathway via an enediol intermediate is shown to be favored in the absence of a metal ion, while the hydride transfer pathway becomes favored in the presence of a metal ion. Our calculations explain why the proton transfer pathway is operative in most aldose-ketose isomerization reactions. These calculations also provide further support for the previously proposed metal ion-mediated hydride transfer mechanism for xylose isomerase.