Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acid resistance in enteric bacteria

J Gorden1, P L Small

  • 1Middlebury College, Vermont 05753.

Infection and Immunity
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Shigella and E. coli survive stomach acid, unlike Salmonella, explaining their low infectious dose. However, acid exposure impairs Shigella

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mycobacterium ulcerans toxin, mycolactone may enhance host-seeking and oviposition behaviour by Aedes aegypti (L.) (Diptera: Culicidae).

Environmental microbiology·2016
Same author

Suspension cultivation of Mycobacterium ulcerans for the production of mycolactones.

FEMS microbiology letters·2001
Same author

Staining of cellular mitochondria with LDS-751.

Journal of immunological methods·2001
Same author

Mycobacterium ulcerans cytotoxicity in an adipose cell model.

Infection and immunity·2001
Same author

Complete structure of the mycolactones.

Journal of the American Chemical Society·2001
Same author

Images in clinical medicine. Massive arteriovenous malformation.

The New England journal of medicine·2001
Same journal

The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

Infection and immunity·2026
Same journal

Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

Infection and immunity·2026
Same journal

<i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

Infection and immunity·2026
Same journal

Group B streptococcal membrane vesicles induce proinflammatory responses in neonatal meninges.

Infection and immunity·2026
Same journal

<i>Pseudomonas aeruginosa</i> infection causes lysosomal dysfunction in the cystic fibrosis bronchial epithelium.

Infection and immunity·2026
Same journal

The role of probiotics in restoring and maintaining vaginal microbiome health: a review.

Infection and immunity·2026
See all related articles

Area of Science:

  • Microbiology
  • Pathogen Survival
  • Gastrointestinal Tract Infections

Background:

  • Shigella species cause dysentery with a remarkably low inoculum.
  • Gastric acid survival is a potential factor contributing to the low infective dose of Shigella.

Purpose of the Study:

  • To investigate the acid tolerance of Shigella species compared to other enteric pathogens.
  • To determine the factors influencing Shigella's acid resistance and its effect on virulence.

Main Methods:

  • Comparative survival assays of Shigella, E. coli, and Salmonella at low pH (2.5) for 2 hours.
  • Assessment of acid survival independence from virulence plasmid and growth temperature.
  • Evaluation of the impact of acid exposure on epithelial cell invasion.

Related Experiment Videos

Main Results:

  • Shigella and E. coli isolates demonstrated significant survival at pH 2.5, while Salmonella isolates did not.
  • Acid tolerance in Shigella was independent of the virulence plasmid and 37°C growth but dependent on growth phase.
  • Acid-exposed Shigella isolates exhibited a diminished capacity for epithelial cell invasion.

Conclusions:

  • Shigella's enhanced gastric acid resistance contributes to its low infectious dose.
  • Acid exposure compromises Shigella's invasive potential, highlighting a trade-off in virulence mechanisms.