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Inosithin neutralization test to measure lupus anticoagulants
R Saxena1, A K Saraya, V K Kotte
1Department of Haematology, All India Institute of Medical Sciences, New Delhi.
American Journal of Clinical Pathology
|January 1, 1993
Summary
The inosithin neutralization test effectively measures lupus anticoagulant levels. This test correlates lupus anticoagulant concentration with thrombosis and recurrent abortion risks, aiding clinical assessment.
Area of Science:
- Hematology
- Immunology
Background:
- Lupus anticoagulant (LA) is associated with adverse pregnancy outcomes and thrombosis.
- Accurate measurement of LA is crucial for risk stratification and management.
Purpose of the Study:
- To evaluate the utility of the inosithin neutralization test for quantifying LA.
- To assess the correlation between LA levels measured by this test and clinical manifestations.
Main Methods:
- The inosithin neutralization test was performed on 14 patients with LA.
- Specificity was tested in patients with hemophilia and Factor VIII inhibitors.
- Kaolin clotting time was used to determine the amount of inosithin required to normalize clotting.
Main Results:
- Inosithin corrected prolonged kaolin clotting time in all LA patients.
- This correction was not observed in hemophilia or Factor VIII inhibitor patients.
- LA concentration, measured by inosithin required for normalization, correlated significantly with thrombosis and/or recurrent abortion.
Conclusions:
- The inosithin neutralization test is a useful method for measuring lupus anticoagulant.
- This test provides a quantifiable measure of LA that correlates with clinical risk.