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A new and rapid method for visualising DNA replication in spread DNA by immunofluorescence detection of incorporated
N Tomilin1, Y u Rosanov, V Zenin
1Institute of Cytology of the Russian Academy of Sciences, St. Petersburg.
Biochemical and Biophysical Research Communications
|January 15, 1993
Abstract:
We have developed a new and rapid immunofluorescent method for visualisation of replicated regions on fixed DNA fibers. Using this method we have found in 5-fluorodeoxyuridine (FrdU)-blocked human cells distinct replication units covering about 60 kb of DNA and corresponding in size to single replicons or chromatin loops. Our results also suggest that nonadjacent replicons within a replicon cluster may be activated after FrdU arrest and that the method may be adapted for localization on fibers of specific DNA sequences.