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Genetically engineered resistance against grapevine chrome mosaic nepovirus
V Brault1, T Candresse, O le Gall
1Station de Pathologie Végétale, INRA, Villenave d'Ornon, France.
Plant Molecular Biology
|January 1, 1993
Summary
Transgenic tobacco plants expressing the grapevine chrome mosaic nepovirus (GCMV) coat protein (CP) demonstrate resistance to GCMV infection. This significantly reduces viral RNA accumulation, offering a novel approach for crop protection against nepoviruses.
Area of Science:
- Plant Virology
- Molecular Biology
- Genetics
Background:
- Nepoviruses are RNA plant viruses impacting perennial crops, transmitted by nematodes.
- Their coat protein (CP) expression in transgenic plants is challenging due to polyprotein processing.
- Grapevine chrome mosaic nepovirus (GCMV) causes significant economic damage.
Purpose of the Study:
- To develop a strategy for expressing GCMV CP in transgenic tobacco.
- To assess the resistance of these transgenic plants against GCMV infection.
- To evaluate the efficacy of CP expression in conferring plant immunity.
Main Methods:
- Construction of a hybrid gene for high-level GCMV CP expression.
- Introduction of the gene into the tobacco plant genome via transformation.
- Inoculation of transgenic and control plants with GCMV and assessment of viral RNA levels.
Main Results:
- Transgenic tobacco plants successfully expressed GCMV CP.
- CP-expressing plants exhibited significant resistance to GCMV.
- Infected transgenic plants showed reduced viral RNA accumulation compared to controls.
- Protection was effective even with purified viral RNA inoculation.
Conclusions:
- High-level expression of GCMV CP in transgenic tobacco confers effective resistance.
- This approach offers a promising strategy for controlling nepovirus diseases in crops.
- Transgenic CP expression can be a viable method for plant disease resistance.