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Determination of ceftibuten in sputum by column-switching high-performance liquid chromatography on-line with
H T Pan1, P Kumari, J A de Silva
1National Yang-Ming Medical College, Department of Pharmacology, Shih-Pai, Taipei, Taiwan, Republic of China.
Abstract:
A column-switching high-performance liquid chromatographic assay on-line with thermospray mass spectrometric detection is described for the determination of ceftibuten, an oral cephalosporin antibiotic, in human sputum. The method does not require sample pretreatment and provides increased selectivity not available from the previously reported method with UV detection. The thermospray mass spectrometric detection conditions were optimized for ceftibuten. The technique has a detection limit of 0.50 micrograms/mL and allows precise, simple, and accurate determination of ceftibuten in sputum over the range 0.50-10.00 micrograms/mL.