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Safe pulmonary preservation for 12 hours with low-potassium-dextran solution
1Department of Cardiothoracic Surgery, University Hospital of Lund, Sweden.
The Annals of Thoracic Surgery
|February 1, 1993
Summary
This study presents a reliable method for evaluating transplanted lung function immediately after surgery. Porcine lungs preserved for 12 hours using Perfadex demonstrated safe function, enabling early weaning from artificial support.
Area of Science:
- Transplantation immunology
- Cardiopulmonary surgery
- Organ preservation
Background:
- Assessing immediate post-transplant lung function is critical.
- Standard lung preservation methods require further evaluation for clinical application.
Purpose of the Study:
- To evaluate the function of porcine left lung allografts after 12-hour preservation.
- To establish a reliable method for continuous functional assessment of transplanted lungs.
Main Methods:
- Porcine left lung allografts were preserved for 12 hours in Perfadex solution.
- Venoarterial extracorporeal membrane oxygenation with a heparin-coated artificial lung was used post-transplantation.
- Right pneumonectomy was performed, and animals were monitored for 24 hours.
Main Results:
- Transplanted lungs maintained good function, with arterial oxygen tension around 200 mm Hg and carbon dioxide tension around 40 mm Hg.
- Animals were successfully weaned from artificial lung support within 1 hour post-pneumonectomy.
- No systemic anticoagulation was required due to the heparin-coated artificial lung.
Conclusions:
- A reliable method for immediate and continuous evaluation of transplanted lung function was demonstrated.
- Low-potassium-dextran solution (Perfadex) enables safe 12-hour lung preservation.
- This approach facilitates early assessment and management of lung allografts post-transplantation.