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Divergent T cell receptor gamma repertoires in rheumatoid arthritis monozygotic twins
H Kohsaka1, P P Chen, A Taniguchi
1Sam and Rose Stein Institute for Research on Aging, University of California, San Diego, La Jolla 92093-0663.
Objective:
To determine if the expressed T cell receptor (TCR) gamma repertoire is altered in rheumatoid arthritis (RA).
Methods:
Peripheral blood lymphocytes were collected from monozygotic twins who were either concordant or discordant for RA, or from a normal twin pair. TCR gamma-specific complementary DNA libraries were constructed using the anchored polymerase chain reaction. Gene usage was analyzed by plaque hybridization and sequencing.
Results:
The expressed TCR V gamma repertoires both in RA patients and normal subjects were extremely diverse. Monozygotic twins who were concordant for RA expressed very different frequencies of TCR V gamma genes.
Conclusion:
RA does not lead to a specific clonal expansion or deletion of TCR V gamma genes in peripheral blood.
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