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In vitro effect of indomethacin on polymorphonuclear leukocyte function in preterm infants
S Kamran1, S S Usmani, R A Wapnir
1Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York 11030.
Pediatric Research
|January 1, 1993
Summary
Indomethacin impairs polymorphonuclear leukocyte (PMN) function, including random motility and chemotaxis. This adverse effect is dose-dependent and more pronounced in preterm infants compared to full-term infants and adults.
Area of Science:
- Immunology
- Pharmacology
- Neonatal Medicine
Background:
- Polymorphonuclear leukocytes (PMN) are crucial for immune response.
- Preterm infants have immature immune systems, increasing susceptibility to infections.
- Non-steroidal anti-inflammatory drugs (NSAIDs) like indomethacin are used in neonates.
Purpose of the Study:
- To investigate the in vitro effects of indomethacin on PMN random motility and chemotaxis.
- To compare the impact of indomethacin on PMN function in preterm infants, full-term infants, and adults.
Main Methods:
- PMN were isolated from cord blood of preterm (<37 wk gestation) and full-term infants, and from adults.
- Isolated PMN were exposed to varying concentrations of indomethacin (0, 300, 600, 900 ng/mL).
- Random motility and chemotaxis assays were performed to assess PMN function.
Main Results:
- Indomethacin significantly decreased PMN random motility and chemotaxis in preterm infants at all tested concentrations in a dose-dependent manner.
- Impairment of PMN function was most significant at 900 ng/mL.
- Full-term infants showed significant impairment in random motility across all concentrations and chemotaxis at 300 and 600 ng/mL compared to adults.
Conclusions:
- Indomethacin adversely affects PMN random motility and chemotaxis.
- The negative impact of indomethacin on PMN function is more severe in preterm infants than in full-term infants or adults.
- These findings highlight potential risks of indomethacin use in neonatal populations, particularly concerning immune function.