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Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods
W H Koch1, W L Payne, B A Wentz
1Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Applied and Environmental Microbiology
|February 1, 1993
Abstract:
The polymerase chain reaction was used to selectively amplify sequences within the cholera toxin operon from Vibrio cholerae O1. Oysters, crabmeat, shrimp, and lettuce were seeded with V. cholerae and then homogenized or washed with alkaline peptone water, followed by short-term (6- to 8-h) enrichment. A detection limit of as few as 1 V. cholerae CFU per 10 g of food was obtained with amplification reactions from crude bacterial lysates. The method is extremely rapid and obviates the need for DNA isolation from a variety of complex food matrices.