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Flow injection cytometry: a new approach for sample and solution handling in flow cytometry
W Lindberg1, J Ruzicka, G D Christian
1Department of Chemistry, University of Washington, Seattle 98195.
Cytometry
|January 1, 1993
Summary
A new modular instrument for flow cytometry automates sample and reagent handling. This system offers precise control over fluidics, enabling accurate on-line staining of cells.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Cell Biology
Background:
- Flow cytometry requires precise sample and reagent handling for accurate cellular analysis.
- Existing methods may lack automation and precise control over fluidic parameters.
Purpose of the Study:
- To develop and evaluate a prototype automated instrument for sample and solution handling in flow cytometry.
- To enable precise control over fluidics for on-line cellular staining applications.
Main Methods:
- Construction of a modular instrument controlled by a computer using TTL-logic.
- Integration with a flow cytometer via a 6-port injection valve for flexible flow pattern implementation.
- Utilized 4 pumps, 2 selection valves, and 2 injection valves for automated fluid control.
Main Results:
- Demonstrated accurate control of injected sample and reagent volumes.
- Achieved reproducible timing and controlled mixing conditions for on-line staining.
- Presented model experiments of on-line trout erythrocyte staining with DAPI and PI.
Conclusions:
- The prototype instrument provides accurate and reproducible sample and reagent handling for flow cytometry.
- The modular design facilitates versatile fluidic control and on-line applications.
- The system shows potential for improving the performance and utility of flow cytometry assays.