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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Published on: December 12, 2015
Evidence for a membrane skeleton in higher plants. A spectrin-like polypeptide co-isolates with rice root plasma
1Section of Plant Biology, Cornell University, Ithaca, NY 14853.
Abstract:
A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M(r) > 100 kDa). One of these polypeptides (M(r) approximately 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.
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