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Related Experiment Videos

Creatine kinase: stability, inactivation, reactivation

L G Morin

    Clinical Chemistry
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    The study determined creatine kinase isoenzyme stability, finding thermal factors drive irreversible inactivation in vivo. Prompt cooling and mercaptoethanol addition are recommended for preserving specimen integrity.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Clinical Chemistry

    Background:

    • Creatine kinase (CK) isoenzymes are crucial biomarkers.
    • Understanding CK isoenzyme stability is vital for accurate clinical diagnostics.
    • In vitro stability data can inform in vivo inactivation mechanisms.

    Purpose of the Study:

    • To determine in vitro biological half-lives of creatine kinase isoenzymes at different temperatures.
    • To elucidate the mechanisms of reversible and irreversible isoenzyme inactivation.
    • To establish optimal conditions for specimen storage and handling.

    Main Methods:

    • In vitro determination of decay constants for CK isoenzymes across various temperatures.
    • Assessment of factors influencing isoenzyme inactivation, including temperature, oxidation-reduction, proteins, urate, catecholamines, dilution, and thiols.

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  • Evaluation of mercaptoethanol as a stabilizing agent for CK isoenzyme MB.

    • Main Results:

    • In vitro half-lives at 37°C align with in vivo data, suggesting thermal inactivation is primary in vivo.
    • Reversible inactivation is linked to oxidation-reduction processes.
    • Proteins and specific inactivators stabilize isoenzymes; dilution and thiols promote reactivation.
    • Mercaptoethanol is optimal for CK isoenzyme MB storage and reactivation.
    • CK isoenzyme MB exhibits sensitivity to light and freeze-thawing.

    Conclusions:

    • In vivo irreversible inactivation of creatine kinase isoenzymes is predominantly driven by thermal factors.
    • Specimen handling protocols including prompt cooling, addition of mercaptoethanol, refrigeration, and avoidance of light/freezing are recommended.
    • A proposed inactivation model involving active, denatured, oxidized, and insulated monomers explains observed phenomena.